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After treatment, each sample (100 L) was mixed with 40 L of SP3 beads mix (20 L of hydrophobic + 20 L of hydrophilic beads), incubated for 15 min and then overlayed with 1 mL of absolute EtOH

Synthetic lethal chemical screens in a panel of lung cancer cell lines and a normal epithelial cell line (BEAS-2B) with small-molecule drugs (n=22) targeting various oncogenic and metabolic pathways ( Supplementary Table 2 ) identified Erastin, an inhibitor of SLC7A11 and potent inducer of ferroptosis by blocking the uptake of cystine for GSH synthesis, that synergized with LDHB-specific siRNAs to suppress the viability of a subset of KRAS -mutant lung cancer cells (A549, H838, H460, H2122, and H2009), despite to a varied degree ( Figure 2a, b

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The paleo approach: Start with bone broth collagen