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For the measurements, yeast cells were grown to midlog phase, 20 OD 600 units of cells (1.5 10 8 cells) were harvested and centrifuged at 4700 g for 2 min at 25C

Possessing 150 times more genes than are found in the human genome, the gut microbiome encodes a vast number of enzymes that function in a variety of metabolic pathways, including the biosynthesis of essential vitamins and the breakdown of complex, non-digestible polysaccharides 1,2,3,4

Stage 2 has fewer levels of B12 and metabolic abnormalities, such as high homocysteine or methylmalonic acid (MMA) levels

10.1161/CIRCRESAHA.117.311401 24 FreitasR

Redox control of antioxidants, metabolism, immunity, and development at the core of stress adaptation of the oyster Crassostrea gigas to the dynamic intertidal environment