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For RPMI 2650 cells, the cytoplasm was highlighted through immunofluorescence (IF) staining of GAPDH using a primary anti-GAPDH mouse monoclonal antibody (Cell Signaling, CST #97166) at a 1:500 dilution in 1% BSA

Baker E, Bubier JA, Reynolds T, Langston MA, Chesler EJ

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