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Cells were then washed with ice cold PBS and lysed with 1X RIPA buffer supplemented with protease inhibitor and phosphatase inhibitor (Life Technologies)

Together, these data indicate that two doses of SpFN induced antibodies that targeted a wide range of neutralizing epitopes with cross-sarbecovirus neutralizing activity, while also facilitating Fc effector functions associated with therapeutic and natural protection

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[DOI] [PMC free article] [PubMed] [Google Scholar] 50.Slifer Z, Pridgen T, Madan J, Krishnan RB, Blikslager AT