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Under normal conditions, NRF2 is recognized and ubiquitinated by the Keap1-Cul3 E3 ligase complex, marking it for proteasomal degradation and maintaining its low levels in the cell

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At 12 hpi, cells were lysed by resuspending in 3 mL of mild lysis buffer (10 mM Tris, pH 7.4, 150 mM NaCl, 2 mM MgCl 2 1 mM EDTA, 1 mM DDT and 0.5 % Igepal) and incubated on ice for 10 min, followed by vortexing for 30 s

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