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Samples in Laemmli buffer were boiled at 95 C for five min and applied to a 15% SDS gel, separated by SDS-PAGE, and stained with coomassie

Following irradiation with the 660 nm laser, the apoptosis rate in the control group remained unchanged, further confirming the biosafety of the laser

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A standard weight loss workup at our clinic includes: Fasting insulin and HOMA-IR, a direct measure of insulin resistance Hemoglobin A1C A full thyroid panel, including Free T3, Free T4, and Reverse T3 (not just TSH) Comprehensive sex hormone panels for both men and women Cortisol patterns Inflammatory markers Advanced lipid particle analysis A DEXA body composition scan to precisely measure fat mass, lean mass, visceral fat, and bone mineral density That diagnostic picture determines the protocol