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Afterward, the membranes were incubated with the appropriate horseradish peroxidase-conjugated secondary antibody and immunoreactive bands were detected by a Fluorchem Imaging System upon staining with ECL Selected Western Blotting Detection Reagent (GE Healthcare, Pittsburgh, PA, USA)

The enhanced glucose homeostasis, which is associated with the intestinal glucotonic effect, was also abolished after inhibition of GLUT1 expression in metformin-treated mice (Fig

This may also be consistent with impaired mitochondrial biogenesis as described in circulating lymphocytes in chronic inflammatory conditions (45) and in patients with idiopathic ME/CFS ( Also, we detected a reduction in serine concentration, which was inversely associated with depression and anxiety scores in LC patients

, which also circumvents the need for derivatization (Vera et al., 2020)