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Where sterile water becomes the wrong choice is in any multi-dose scenario without a preservative, each additional needle insertion introduces contamination risk that compounds over time

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Psychological stress raises cortisol and adrenaline, which increase muscle tone and reduce the threshold for spontaneous contraction

Interestingly, the presence and/or reorganization of sensory terminal afferents in Negative Control animals is accompanied by sensory behavioral recovery, albeit delayed compared with that seen in PEG-fused animals (see companion paper