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Genes for antibody domains were synthesized by Geneart (Regensburg, Germany) and cloned into the pET28b vector (KanR) via the XbaI/Xho restriction sites keeping the sequence between XbaI and NcoI at the 5 end of the nucleotide sequence of the proteins using sequence and ligase- independent cloning 47,48

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Biochem Eng J 182:108418 Jiang L, Guan X, Liu H, Chang X, Sun J, Sun C, Zhao C (2023) Improved production of recombinant carboxylesterase FumDM by co-expressing molecular chaperones in Pichia pastoris

succinogenes was amplified through PCR using pET28a- as as the template

Second, some evidence suggests it may have modest direct effects on migraine symptoms, though data here are limited