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ZhaoJHuangWZhangSXuJXueWHeBet al

(2020) 159:12901301.e5

After removal of the overlying meninges and blood vessels, spinal cords were cut into small pieces, dissociated with 0.05% collagenase I (Thermo Fisher Scientific, Foster City, CA, USA) for 15 min at 37 C, and then processed to produce epSPC neurospheres as previously described 6,18

This type of referred pain is also vague and ill-definedfelt in the mid-thoracic/posterior scapulae region with herniated cervical disks and in the lumbosacral/buttock region with herniated lumbar disks