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Graphical abstract Table 1

10.1056/NEJMoa2031054 184

Organoid preparation and culture To generate gastric cancer organoids, fresh tumor tissues were transported on ice in cold DMEM/F12 (Gibco) with primocin (InvivoGen), then washed in cold PBS, minced into fragments (1 mm), and digested for 1 h at 37 C with collagenase and hyaluronidase

In addition, through the detection of enzyme-linked immunosorbent assay (ELISA), MnP + dsDNA induced the overexpression of Ifn-I, which was a marker of activation of the cGAS-STING signaling pathway (Fig