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Unlike many research peptides that originate in de novo pharmacology programs, BPC-157 arose from a biological observation: a protein found naturally in the gastric environment appeared to have protective effects on gastric mucosa, and sequencing work identified the 15-amino-acid fragment that retained much of the protective activity of the parent molecule

Such high micromolar GSSG concentrations are unlikely to occur in most subcellular compartments but could be reached in the secretory pathway or during titration experiments of transgenic cell lines with an upregulated GSSG transporter 35,63,64,77

doi: 10.1002/14651858.CD007137.pub4 58 PieperR.Scharek-TedinL.ZetzscheA.RheI.KrgerS.VahjenW.et al

These features limit the use of GST for oligomeric protein tagging and for studying proteinprotein interactions under oxidative stress conditions