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Western blot analysis To assess protein expression, cells were pelleted at 10,000 g for 2 min and resuspended in B-PER (Thermo Scientific) lysis buffer (supplemented with 100 g/mL lysozyme, 20 g/mL DNase I, and 20 g/mL RNase A) according to the equation: volume of lysis buffer (L) = volume of culture (mL) x OD 600 of culture x 100

It might lead to scarring, compromised vascular supply, and impaired cell niches necessary to support sperm or follicles

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Tectoridin (25, 50 and 100 mg/kg), an isoflavone glycoside from the flower of pueraria, could significantly reduce the levels of serum alanine transaminase (ALT), aspartate aminotransferase (AST), and triglyceride (TG) by dose-manner, meanwhile reverse alcoholic steatosis in mice oral administrated with ethanol (5 g/kg), through reversing the decrease in the levels of medium-chain acyl-CoA dehydrogenase, acyl-CoA oxidase and cytochrome P450 4A in peroxisome proliferators-activated receptor (PPAR-) and its related target genes [280]