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The study found that ME/CFS patients have significantly elevated IgA and IgM antibodies directed against the lipopolysaccharides (LPS) of enterobacteria, proving the presence of "leaky gut." Crucially, the researchers demonstrated that when these patients were treated with specific natural anti-inflammatory substancesprimarily L-Glutamine, combined with N-acetyl cysteine (NAC) and zinc, in conjunction with a leaky gut diet for 10-14 monthsthe leaky gut barrier was restored

Western blot analysis For protein analysis, cells or pieces of liver tissue were washed in PBS and lysed in Lysis Buffer (20 mM Tris-HCl pH8, 1 mM NaCl, 0.5% NP40, 0.5% Triton-X-100, 10% glycerol) supplemented with a protease inhibitor cocktail (SigmaAldrich)