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Not only surface markers but also certain histologically stained intracellular components can be regarded as activation markers, since activated eosinophils undergo degranulation and release granule proteins detectable by specific immunostaining

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L., Daz-Jimenez, J., et al

These ASD animal models are characterized by analogous behavioral deficits to those observed in patients and are useful in investigating novel genetic risk factors [18]

After 48 h, biofilms were washed once with 1 PBS followed by treatment with different enzyme solutions prepared in 1 PBS, pH 7.4, including: 5, 10, 20, and 40U DNase-I, 100, 200, 500 and 1000 g/ml amylase and 50, 100, 200 and 500 g/ml Proteinase K, for 24 h, at 37C and 150 rpm, the control was treated with 1 PBS alone