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Indeed, many of the traditional CVD risk factors, including oxidized low-density lipoprotein[313], hyperlipidemia (hypercholesterolemia or mixed dyslipidemia)[314] insulin resistance[309, 310, 312], diabetes[315], obesity[309, 311], elevated C-reactive protein[316], waist-to-hip ratio[309], reduced coronary flow reserve[279], smoking[317], sedentary life[318] and psychological distress[319], which act via effects on endothelial dysfunction, apoptosis and impaired endothelial-dependent vascular reactivity, have been associated with low serum IGF-I levels and reduced IGF-I and IGF-IR mRNA as well as protein expression in vascular smooth muscle cells (VSMC)[313]

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Generally, it performs best in aqueous systems within a pH range of 5.0 to 7.0

Endpoints frequently measured include the activity of alkaline phosphatase (ALP), an early marker of osteoblast differentiation, the expression of the master osteogenic transcription factor Runx2, and the deposition of a mineralized matrix, which can be visualized with Alizarin Red staining