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Acipayam, C

Table 25: its express by 100 M Q10 regulate from the gene of apoptosis array and the tabulation of their possible functions in cell Raise (Lycoperdon polymorphum Vitt) and downward modulation (white) Embodiment 12: about the PCR diabetes array of hepatocarcinoma (HepG2) cell The thing that utilizes the medium processing HepG2 (hepatocarcinoma) cell carried out 24 hours or utilized 100 MQ10 to handle said cell and carry out different time.According to the method that is used for the PaCa2 cell (preceding text, embodiment 9-11) with 1 * 10 5 Individual cells/well initiated process.Yet, be lower than expection from the total amount of the RNA of these sample extraction.Usually use total RNA (measuring) of 1 g to carry out reverse transcription through the measurement at 260nm place.The spendable maximum volume of every reverse transcription is 8 L.Because RNA concentration is lower, so use utilize the medium thing and of the RNA of 0.44 g from 16 hours RT-PCR array analysis with the sample of 48 hours Q10 processing.Said array provides the trend and the preliminary analysis of pattern of the HepG2 Gene regulation of utilizing 100 M Q10 to handle to carry out, like what summarize in the following table 26.Said result shows each the quilt downward modulation (about respectively 1/20,1/6 and 1/5) in the 16th hour after processing among gene PPARGC1A, PRKAA1 and the SNAP25.After processing the 48th hour, PPARGC1A and PRKAA1 standardization or raised slightly, however SNAP25 is reduced about 1/2

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