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Adding higher or lower concentration of standard may cause inconsistent signal or false positive

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In the presence of mitochondrial superoxide dismutase (SOD), O 2 is converted into H 2 O 2 , which then diffuses from mitochondria into the cytosol

doi: 10.1038/s41418-022-01003-1 205 ZoghbiHYBeaudet al

For instance, in glucose-deficient glioblastoma, heightened SLC7A11 expression leads to the generation of ROS and oxidative stress through the consumption of intracellular NADPH during the conversion of imported L-cystine to L-cysteine ( in vivo , within a tumor microenvironment, where the metabolic reprogramming associated with altered SLC7A11 expression was evident ( in vivo experiments demonstrated that heightened SLC7A11 expression facilitated localized tumor growth while impeding tumor migration