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Hold the syringe needle-up and tap gently to move any remaining bubbles toward the needle

A GHK-Cu preparation with incorrect copper stoichiometry (e.g., under-complexed peptide with free tripeptide present) will clear faster and bind tissue more weakly than properly synthesized material

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Next, we employed a fluorescence polarization assay (FPA) to pinpoint the sequences crucial for binding among the specified regions, subsequently improving the cell applicability of the peptide sequence