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RMSF and solvent-accessible surface area (SASA) (Tabrez et al., 2022)

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demonstrated that Erastin treatment in fibroblasts and fibrosarcoma cells robustly induced the conversion of LC3I to LC3II and the formation of GFP-LC3 puncta, which are definitive hallmarks of autophagy activation (49)

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Using the approach of Habig et al

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