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Adherent glial cells were washed in PBS and incubated at 37C with 1:5 dilution of 10 Trypsin-EDTA (Sigma-Aldrich) in high glucose DMEM until astrocyte detachment was complete (25 min)

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Methionine: An essential amino acid that aids in breaking down fats, preventing cholesterol buildup, and eliminating heavy metals from the body, which can cause kidney dysfunction, nervous system disorders, and immune system issues