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Diminished GST activity was also noted in the lung and liver tissues of rats treated with an extract of chewing tobacco.[] An increase in the activity of cytochrome P450 and cytochrome b5 enzymes was observed in the liver of mice fed an extract of chewing tobacco by gavage.[] Urine samples from females who used masheri or masheri 0 + BQT were mutagenic in Salmonella typhimurium strain TA 100 directly and upon nitrosation with sodium nitrite,[] while, in another study, samples from masheri habitus were mutagenic in strains TA 98 and TA 102 upon metabolic activation with rat liver microsomal fraction (S9).[] Gastric fluid from chewers of tobacco and lime was directly mutagenic in S