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The route of administration, dosage, and injection technique all influence potential side effects

(Injecting plain water in large volume would cause cells to swell and rupture due to osmotic imbalance, so sterile water is only given after adding an appropriate amount of solute to make it nearly isotonic

Due to the existence of a reservoir that is efficiently used by the enterohepatic circuit, a deficiency due to dietary absence of B12 may take some time to appear if reserves are adequate at the time the vitamin supply ceases

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Experimental studies typically examine receptor-independent and receptor-associated signalling effects, transcriptional responses, and peptide stability under controlled conditions