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Walker A, Chapin B, Abisambra J, DeKosky ST

Construction of the stable transgenic C32E8.9 OE strain To generate a stable overexpression strain of the C32E8.9 gene in C

Each wash should also be analyzed to observe any eluted protein, and bait- and prey-free controls should also be used to confirm that there are no bait-prey interactions in the bait lysate and that the prey protein does not bind to the immobilized support.