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Following initial homologous recombination, enrichment of PrfA* (G145S) mutants was by performed by passaging merodiploid strains at 30C shaking in selective Listeria Synthetic Media (LSM) with 55mM glucose-6-phosphate without glutathione (Sigma-Aldrich G7879) and plated on p-chlorophenylalanine as a counter selectable marker

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Natural Glutathione is an antioxidant that the body naturally produces and is often discussed in relation to cellular balance

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A Review on the Innate Immune Memory and its Potential Role in Infections, Diseases and New Therapeutic Strategies