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A 15-nt elongation product is detected with the 3 vRNA-1 template (lane 2, orange arrow), while the control reaction yielded a 9-nt product (lane 1, red arrow)

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Downregulation of PCBP1/2 mediates Fe 2+ release and induces ferroptosis To explore the potential mechanism by which ACE elevates Fe 2+ levels, iron metabolism proteins associated with ACE were screened by Western blot based on proteomic results (Fig