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A total of 48 h after transfection, cells were collected and RNA was extracted using the RNeasy Mini Kit (Qiagen, 74106), according to the manufacturers instructions

Nanomedicine .2012;8:20411

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Many lifestyle factors also contribute to insomnia, including stress, poor sleep habits, and travel

The data generated during this phase help define the dose range that may be explored in subsequent studies and contribute to understanding exposure-response relationships, pharmacokinetic behavior and safety considerations associated with the investigational compound