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In PD, researchers are examining if gut microbiome composition associates with markers like alpha-synuclein in colonic biopsies or with REM sleep behavior disorder severity (a prodromal feature)

Biochim Biophys Acta 1840:906912 Liddell JR, Robinson SR, Dringen R, Bishop GM (2010) Astrocytes retain their antioxidant capacity into advanced old age

The purified DNA was digested into component nucleotides using nuclease P 1 , snake venom phosphodiesterase and alkaline phosphatase as previously described in detail

For peptides that do not dissolve cleanly in bacteriostatic water alone typically those with hydrophobic sequences or basic amino acid-rich compositions that require protonation for solubilisation the recommended approach is to first perform initial reconstitution in a small volume of 0.1% acetic acid water (also available from Peptides Lab UK) to produce a clear stock solution, before diluting further with bacteriostatic water or the relevant physiological buffer