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By preventing nicotinamide methylation, the compound increases intracellular NAD+ availability, which in turn activates sirtuins and other NAD+-dependent enzymes critical for metabolic homeostasis

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The purity of cytosolic and mitochondrial fractions was validated by Western blotting using anti-GAPDH and anti-VDAC antibodies, respectively

[DOI] [PMC free article] [PubMed] [Google Scholar] 5.Soler AP, Miller RD, Laughlin KV, Carp NZ, Klurfeld DM, Mullin JM