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Intermittent high glucose-induced oxidative stress modulates retinal pigmented epithelial cell autophagy and promotes cell survival via increased HMGB1

Additionally, curcuminoids can be quantified using UV-VIS if they absorb within the matrix or sample components

S., Rao, Y., & Zhou, X

Leaf samples were collected at 1, 5, 10, 15, and 18 days after stress treatment, rinsed with RNase-free water, flash-frozen in liquid nitrogen, and stored at 80C until further analysis

A sensible experiment spreads doses across at least three orders of magnitude, something like 1, 10, and 100 nanomolar on up to 1 and 10 micromolar, precisely because the sweet spot moves with the copper-to-peptide ratio, the buffer, and how much serum sits in the media