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Vitamin B12 deficiency is a genuine clinical concern but the right treatment depends on accurate diagnosis and individual factors

Hunter et al., 2003

Proteins were detected on nitrocellulose membranes (Bio-Rad, #1620115) using the following primary antibodies: 6*His-tag (clone 4A4 or 2F12, source: antibody facility of the Helmholtz Center Munich, hybridoma supernatants were used at a 1:10 dilution) 75 , -TRAF6 (H-274, Santa Cruz Biotech., #sc-7221, used at 1:1000), -HA-tag (12CA5, Sigma-Aldrich, #11583816001, used at 0.4 g per mL), -IB (C-21, Santa Cruz Biotech., #sc-371, used at 1:500) and -Tubulin (B-5-1-2, Santa Cruz Biotech., #sc-23948, used at 1:500)