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Measurement of ROS production Cells were washed in prewarmed PBS and treated with 10 M H 2 DCFDA for 30 min in dark

Rather than viewing depletion as a single-variable issue, the session emphasized glutathione status as reflective of overall intracellular stress exposure

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IV fluids replace lost plasma volume and restore sodium, potassium, and other electrolytes more quickly than drinking alone useful after heavy sweat losses or prolonged events

After 2 h treatment period, the mixtures were centrifuged at 10,000 g for 1 min to precipitate the erythrocytes