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The homogenate was filtered through a 100-M cell strainer and centrifuged at 1,000 g for 10 min at 4 C, then the nuclear pellet was washed once in nucleus preparation buffer followed by resuspension in PBS with 1% BSA and 0.2 U l Roche Protector RNase inhibitor

A meta-analysis of 27 studies ( n = 971 BD patients) showed higher DNA and ribonucleic acid (RNA) damage along with increased lipid peroxidation and nitric oxide levels, a potent free radical, which implies oxidative stress and the potential failure of antioxidant defenses [155]

60 This study also measured glutamate reuptake from forebrains of astrocyte or neuron EAAT2 knockout mice reconstituted into liposomes, to account for inefficient astrocyte membrane resealing during synaptosome preparation

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However, you should consult your doctor before taking any supplements, especially if you have any medical conditions or are taking any medications