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Scenario B (More Diluted): You add 2ml of BAC water

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When your target is 25 units (2.5 mg at 10 mg/mL), reading the 25th line on a 0.5 mL syringe is much easier and more accurate than trying to hit the midpoint between 24 and 26 on a 1 mL syringe where lines are spaced closer together

As the value become more negative it will show more adhesion with the biology membrane

In Figures 3 and 4, the cluster of three charged residues consists of Arg123, Arg127 and Glu40, where the two arginines possess positively charged side chains, while the glutamate (Glu40) possesses a negatively charged side chain