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132 KawaiH.IwadateR.IshibashiT.KudoN.KawashimaY.MitsumotoA

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Activation of the phosphatidylinositol 3-kinase/protein kinase Akt pathway mediates nitric oxide-induced endothelial cell migration and angiogenesis

Kress CL, Konopleva M, Martnez-Garca V, Krajewska M, Lefebvre S, Hyer ML, McQueen T, Andreeff M , Reed JC, Zapata JM

Immunofluorescence analysis HGC27 cells with indicated treatments were fixed with 4% paraformaldehyde for 15 min, permeabilized with 0.2% Triton X-100 for 10 min, and blocked with blocking buffer containing 1% BSA for 1 h at room temperature