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This assay is based on the ability of the MDA to react with the thiobarbituric acid (TBA) under acidic condition, leading to the formation of MDA-TBA2 adducts called TBARS

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This was most recently shown by such studies as ASCEND (A Study of Cardiovascular Events in Diabetes) [71], STRENGTH (Long-Term Outcomes Study to Assess Statin Residual Risk with Epanova in High Cardiovascular Risk Patients with Hypertriglyceridemia) [72], OMEMI (Omega-3 fatty acids in Elderly with Myocardial Infarction) [73] and VITAL (Vitamin D and Omega-3 Trial) trials [74]

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Choudhury R, Bonacci T, Wang X, Truong A, Arceci A, Zhang Y, et al