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GPCRs play a crucial role in mediating cellular responses to a wide variety of extracellular signals, including neurotransmitter molecules and hormones, which activate G proteins

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In persons with intestinal lesions such as jejunal diverticulosis or some anatomical abnormalities with bacterial overgrowth (e.g., fistulas and blind-loops), vitamin B12 deficiency may develop because bacteria in the colon competitively utilize all the available vitamin B12 (Cooke et al., 1963)

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This subcutaneous chemical irritant activates the peripheral nerves, leading to the activation of dorsal horn neurons