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Finally, 2 l 30% glycerol (in reservoir solution) was added to the drop before picking the crystal using 0.1 mm litholoops and flash-freezing it in liquid nitrogen

* Correspondence: Ayodeji Mathias Adegoke, [email protected] Disclaimer All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers

Move the plunger in and out several times while tapping the syringe, thus removing all air bubbles