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IGF-1 Regulation of Type II Collagen and MMP-13 Expression in Rat Endplate Chondrocytes via Distinct Signaling Pathways

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or (2) R 7 and R 8 may together be alkylene or alkenylene, completing a 3-to 8-membered saturated or unsaturated ring with the nitrogen atom to which they are attached, which ring is unsubstituted or substituted with Z 1 , Z 2 and Z 3

To monitor cell viability, the cells were trypsinized, and apoptosis/necrosis induced by pneumococcal infection was evaluated using flow cytometry with an Annexin V/propidium iodide labeling kit (Invitrogen), showing 5% across all time points