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To confirm that this replication restart mechanism is involved in the unrestrained replication fork progression observed in HRASV12- and cyclin E-overexpressing cells upon inactivation of fork reversal, we carried out a modified DNA fiber assay where cells were permeabilized after CldU/IdU labeling and then treated with S1 nuclease, which specifically cleaves single-stranded nucleic acids, including single-stranded regions of duplex DNA 41,42

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