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C.PinnaG.ZhubiA.MalokuE.VeldicM.CostaE.et al (2006)

Following a 24 h-treatment, cells were washed once with 1 PBS and incubated for 2 h in a saline buffer (139 mM NaCl, 0.56 mM MgCl 2 , 10 mM Hepes, 2.7 mM KCl, 1 mM K 2 HPO 4 , 1.8 mM CaCl 2 pH7.4 supplemented with 10 mM glucose) containing 100 M of 7-Azido-4-Methylcoumarin (AzMC) fluorescent probe (Sigma Aldrich), which selectively reacts with H 2 S to form a fluorescent compound

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It is a chronic autoimmune disorder that affects the central nervous system, leading to inflammation and damage to the myelin sheath that surrounds nerve fibers in the brain and spinal cord

Diluting the reconstituted solution with additional bacteriostatic water (30-90 units) reduces this irritation