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The aim of this analysis was to study the association between ballooning reduction and steatosis reduction in the resmetirom versus placebo group using second harmonic generation (SHG)/two-photon excited fluorescence (TPEF) microscopy imaging of paired biopsy samples with artificial intelligence (AI)-based algorithms

A comparison of our methodology and previously published assays has been presented in Tables 3 and 4

Nine main cell types were identified and used for differential gene transcription within the individual clusters (Figure 3B and Supplemental Figure 2)

Transmission electron microscopy Cells were digested with 0.25% trypsin and 0.02% EDTA, washed with Hanks Balanced Salt Solution (HBSS)