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HEK-293 cells were incubated in DMEM medium supplemented with 10% FBS and 1% antibiotics at 37 C under 5% CO 2

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doi: 10.1007/s00394-020-02314-0 20 BirkelandEGharagozlianSBirkelandKIValeurJMageIRudIet al

With the help of the enzymes hexokinase, phosphofructokinase, 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase-3 (PFKFB3) and pyruvate kinase, glycolysis rapidly converts a single molecule of glucose into two molecules of pyruvate with a net gain of two ATP molecules and two molecules of NADH

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