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Determination of Reduced (GSH) and Oxidized (GSSG) Glutathione Plant tissue extraction and analyses of GSH and GSSG were carried out as described by HT autosampler, a CTO-20A column thermostat, an SPD-M20A UV/VIS photodiode array detector, and an LCMS 2020 single quadrupole mass spectrometer equipped with an ESI sample pulverization unit

Glutamine is recognized as an indirect antioxidant due to its role in glutathione synthesis, which aids in the elimination of ROS and shields against muscle damage [145]

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Glutathione is your body's master antioxidant, but every time it neutralises a free radical, it gets "used up" chemically oxidised, less active, waiting to be recycled