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A secondary metabolite gliotoxin in A.fumigatus hampers the ROS production by the neutrophils (252)

The constructs were amplified from the previously described hTrxR1 U498C construct, using OhTrxRf (5-TATTACATATGAACGGCCCTGAAGATCTTCCCAAGTCC-3) as forward primer, and OhTrxRrSC (5-TATTAAAGCTTTTAACCACAGGAGCCAGCCTGGAGGAT-3), OhTrxRrCS (5-TATTAAAGCTTTTAACCAGAGCAGCCAGCCTGGAGGAT-3), or OhTrxRrCtrunc (5-TATTAAAGCTTTTAACCTTAGCAGCCAGCCTGGAGGAT-3) as reverse primers for PCR, respectively 33

Hub genes within this module, along with variably expressed transcription factors, provide strong candidates for future validation

The frequency of these injections at our clinic can greatly vary, depending on several factors