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These modifications occur at four key positions: position 2 prevents DPP-IV cleavage, position 8 reduces asparagine rearrangement, position 15 enhances bioactivity, and position 27 prevents methionine oxidation

Notably, the methylation levels of certain genes are significantly correlated with age of onset and disease duration

We also analyzed ChIP-seq datasets of histone H3 modifications, including H3K4me3, H3K4me1, H3K27ac, and H3K27me3, previously generated by us from human HEK293T cells 27

The in vivo spin trapping and electron paramagnetic resonance (EPR) spectroscopy revealed that cysteine deficiency significantly increased lipid-derived radicals in BAT with undetectable signals in white adipose tissue (WAT) (Fig