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4.3.2 Function of curcumin in ALS Curcumin is recommended as a dietary supplement to reduce the risk of ALS (McCombe and Henderson, 2010)

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RT-PCR was done using a standard SYBR-green PCR kit (SYBR Premix EX TaqTM II, Takara), and the gene-specific PCR amplification was conducted using the Applied Biosystems StepOnePlus Real-Time PCR System (Applied Biosystems, USA)