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Cells were treated with PEMS buffer containing 1% Triton X-100 at room temperature for 1 min, washed twice with PEM buffer, and incubated in PEMBAL buffer (100 mM PIPES pH 6.9, 1 mM MgCl 2 , 1 mM EGTA, 1 % BSA, and 0.1 M L-lysine) at room temperature for 1 h with gentle rotation

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Cancer cells may even rewire metabolic pathways, usually by enhancing glycolysis (known as Warburg effect) and glutaminolysis, to sustain higher rate of ATP production or energy supply [1, 4, 6]

Common symptoms of B12 deficiency include fatigue, weakness, memory issues, and mood changes

E.et al (2010b)