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D.HayanoM.et al (2014)

Endocrinology 130:26972702 Mulroney SE, Haramati A, Werner H, Bondy C, Roberts CT Jr, LeRoith D (1992) Altered expression of insulin-like growth factor-I (IGF-I) and IGF receptor genes after unilateral nephrectomy in immature rats

Consistency over several months is usually required to see a meaningful difference

Additionally, in 2D cultures, ECs can easily spread and migrate due to the lack of physical impediments, while in 3D cultures, a surrounding matrix poses constraints that enforce the ECs adaptation to fit through matrix pores and often require matrix degradation for spreading and migration [240]