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20 mM Tris-HCl, pH 7.5, 1% Triton X-100, 10% glycerol, 132 mM NaCl, Roche complete protease inhibitor ) or in RIPA (for PP2A), supplemented with Roche complete protease inhibitor)

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Survival data was fitted with Gompertz mortality rate function adjusted for left truncation with flexsurvreg function from flexsurv R package 206 : Mean and standard error estimates of parameters ln ( A ) and r from individual mortality curves corresponding to the same experimental model (strain, sex, intervention) were used to calculate aggregated meta-parameters

In other words, they initiate free-radical reactions without being used up during the reaction

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